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Cis-mediated down-regulation of a trypsin gene associated with Bt resistance in cotton bollworm
Liu, Chenxi1; Xiao, Yutao1; Li, Xianchun2; Oppert, Brenda3; Tabashnik, Bruce E.2; Wu, Kongming1
2014
发表期刊SCIENTIFIC REPORTS
ISSN2045-2322
卷号4页码:-
摘要Transgenic plants producing insecticidal proteins from the bacterium Bacillus thuringiensis (Bt) are useful for pest control, but their efficacy is reduced when pests evolve resistance. Here we examined the mechanism of resistance to Bt toxin Cry1Ac in the laboratory-selected LF5 strain of the cotton bollworm, Helicoverpa armigera. This strain had 110-fold resistance to Cry1Ac protoxin and 39-fold resistance to Cry1Ac activated toxin. Evaluation of five trypsin genes revealed 99% reduced transcription of one trypsin gene (HaTryR) was associated with resistance. Silencing of this gene with RNA interference in susceptible larvae increased their survival on diets containing Cry1Ac. Bioassays of progeny from crosses revealed that resistance to Cry1Ac was genetically linked with HaTryR. We identified mutations in the promoter region of HaTryR in the resistant strain. In transfected insect cell lines, transcription was lower when driven by the resistant promoter compared with the susceptible promoter, implicating cis-mediated down-regulation of HaTryR transcription as a mechanism of resistance. The results suggest that H. armigera can adapt to Bt toxin Cry1Ac by decreased expression of trypsin. Because trypsin activation of protoxin is a critical step in toxicity, transgenic plants with activated toxins rather than protoxins might increase the durability of Bt crops.
学科领域Multidisciplinary Sciences
DOI10.1038/srep07219
收录类别SCI
语种英语
WOS记录号WOS:000346252800002
出版者NATURE PUBLISHING GROUP
nyguidd55cefb5-7f78-4b9e-b6db-4b0631318a88
引用统计
文献类型期刊论文
条目标识符http://ir.nais.net.cn/handle/2HMLN22E/264289
专题植物保护研究所_有害生物天敌研究室
院机关
作者单位1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China;
2.Univ Arizona, Dept Entomol, Tucson, AZ 85721 USA;
3.USDA ARS, Ctr Grain & Anim Hlth Res, Manhattan, KS 66502 USA
第一作者单位植物保护研究所
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GB/T 7714
Liu, Chenxi,Xiao, Yutao,Li, Xianchun,et al. Cis-mediated down-regulation of a trypsin gene associated with Bt resistance in cotton bollworm[J]. SCIENTIFIC REPORTS,2014,4:-.
APA Liu, Chenxi,Xiao, Yutao,Li, Xianchun,Oppert, Brenda,Tabashnik, Bruce E.,&Wu, Kongming.(2014).Cis-mediated down-regulation of a trypsin gene associated with Bt resistance in cotton bollworm.SCIENTIFIC REPORTS,4,-.
MLA Liu, Chenxi,et al."Cis-mediated down-regulation of a trypsin gene associated with Bt resistance in cotton bollworm".SCIENTIFIC REPORTS 4(2014):-.
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